Ⅱ型糖尿病患者与健康男性血清中19种微量元素含量对比
Concentrations of 19 Trace Elements in Type Ⅱ Diabetes Compared with Healthy Male Controls
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摘要: 微量元素代谢在糖尿病患者中可能发生了改变，这些元素在糖尿病发病机制和发展中起特定的作用。本工作研究了年龄区间50~60岁男性Ⅱ型糖尿病患者（95例）与健康人对照组（91例）的血清微量元素含量的差异。采用微波消解法对血清中19种微量元素进行前处理，稀释25倍，选择电感耦合等离子体质谱法（ICP-MS）进行检测。该方法的检测限、精密度和重复性良好，符合血清微量元素的检测要求。相比于对照组，糖尿病患者血清中锰、钴、铜、钡和铅元素的含量相对较高，钒、锌、砷、锑和钨元素的含量相对较低，而铬、铁、镍、硒、钼、镉、锡、汞和铊元素的含量在糖尿病患者和健康对照组中无显著统计学差异。结果表明，与糖尿病密切相关的10种微量元素在年龄区间50~60岁男性糖尿病患者与健康人之间存在显著统计学差异。该结论可为进一步研究与微量元素缺乏或者过量相关的糖尿病发病机制和发展过程提供数据支持。
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关键词:
- 微量元素 /
- 糖尿病 /
- 血清 /
- 电感耦合等离子体质谱（ICP-MS）
Abstract: There are increasing evidences showing that the metabolism of trace elements in diabetic patients has being varied. These elements might play some specific roles in the pathogenesis of diabetes and its development. The differences of human serum trace element concentration between age range of 50-60 years old male with type 2 diabetes (95 cases) and healthy control group (91 cases) were studied. Concentrations of 19 trace elements in the serum samples were determined with acid assist microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS) platform with a dilution factor of 25. The detection limit, precision and reproducibility were proved sufficient to serum trace element measurement. Compared with the control group, the patients of diabetes have higher concentration of elements Mn, Co, Cu, Ba, Pb and have lower concentration of elements V, Zn, As, Sb, W, there is no significant statistical differences between the diabetic patients and the healthy control group of elements Cr, Fe, Ni, Se, Mo, Cd, Sn, Hg, Tl. The results show that there are 10 trace elements which closely related to the diabetes mellitus have significant statistical concentration differences between the diabetic patients and the healthy control group with the age range of 50-60 years old male. This conclusion is helpful to study further on the pathogenesis of diabetes-related diseases which related on excess or deficiency of trace elements in the human serum, and also offer data support to further elaborate mechanisms and the development of diabetes which related with trace elements in the human serum. -
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